首頁» 新聞動態» 最新論文

GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins

  GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins

  作者:Yulong Ren, Yihua Wang, Tian Pan, Yunlong Wang, Yongfei Wang, Lu Gan, Zhongyan Wei, Fan Wang, Mingming Wu, Ruonan Jing, Jiachang Wang, Gexing Wan, Xiuhao Bao, Binglei Zhang, Pengcheng Zhang, Yu Zhang, Yi Ji, Cailin Lei, Xin Zhang, Zhijun Cheng, Qibing Lin, Shanshan Zhu, Zhichao Zhao, Jie Wang, Chuanyin Wu, Lijuan Qiu, Haiyang Wang, Jianmin Wan*


  刊物名稱: The Plant Cell

  出版年份:January 2020

  doi: 10.1105/tpc.19.00863


  Dense vesicles (DVs) are vesicular carriers, unique to plants, that mediate post-Golgi trafficking of storage proteins to protein storage vacuoles (PSVs) in seeds. However, the molecular mechanisms regulating the directional targeting of DVs to PSVs remains elusive. Here, we show that the rice (Oryza sativa) glutelin precursor accumulation5 (gpa5) mutant is defective in directional targeting of DVs to PSVs, resulting in discharge of its cargo proteins into the extracellular space. Molecular cloning revealed that GPA5 encodes a plant-unique phox-homology domain-containing protein homologous to Arabidopsis thaliana ENDOSOMAL RAB EFFECTOR WITH PX-DOMAIN (EREX). We show that GPA5 is a membrane-associated protein capable of forming homodimers and that it is specifically localized to DVs in developing endosperm. Colocalization, biochemical, and genetic evidence demonstrate that GPA5 acts in concert with Rab5a and VPS9a to regulate DV-mediated post-Golgi trafficking to PSVs. Furthermore, we demonstrated that GPA5 physically interacts with a class C core vacuole/endosome tethering (CORVET) complex and a seed plant-specific VAMP727-containing R-soluble NSF attachment protein receptor (SNARE) complex. Collectively, our results suggest that GPA5 functions as a plant-specific effector of Rab5a required for mediating tethering and membrane fusion of DVs with PSVs in rice endosperm.


TOP 分分pk10-专业购彩 岐山县 | 突泉县 | 宜州市 | 株洲市 | 三原县 | 恩平市 | 永靖县 | 新密市 | 宁乡县 | 简阳市 | 金塔县 | 岳池县 | 江安县 | 汝州市 | 麦盖提县 | 石泉县 | 永城市 | 怀仁县 | 凤城市 | 隆子县 | 丰都县 | 大庆市 | 三门县 | 隆德县 | 云安县 | 肃南 | 大理市 | 汝阳县 | 兴仁县 | 丰县 | 北宁市 | 泗阳县 | 吉林市 | 扬中市 |